G9545
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 | Immunoblotting Whole mouse 3T3 (A) or human HeLa (B) cell extracts were separated on SDS-PAGE and blotted with Rabbit Anti-GAPDH (Cat. No. G9545). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody 0.1 μg/mL 2: Antibody 0.1 μg/mL + GAPDH immunizing peptide (mouse 314-333) |
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G9545
Sigma
Anti-GAPDH antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-38-kD component, Anti-G3PD, Anti-GAPD, Anti-Glyceraldehyde-3-phosphate dehydrogenase, Anti-OCAS, Anti-OCT1 coactivator in S phase, Anti-p38 component |
Description
| Immunogen | synthetic peptide corresponding to amino acids 314-333 of mouse GAPDH, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in rat and differs by two amino acids in human. |
| Physical form | Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~36 kDa |
| species reactivity | rat, human, mouse |
| concentration | ~1 mg/mL |
| application(s) | immunoblotting: 0.1-0.2 μg/mL using whole extract of human HeLa cells |
| immunoprecipitation: 5-10 μg using mouse NIH3T3 cell lysates | |
| indirect immunofluorescence: 5-10 μg/mL using rat NRK cells | |
| Gene Information | human ... GAPDH(2597) |
| rat ... Gapdh(24383) | |
| mouse ... Gapdh(14433) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | Aldridge, G., et al., The use of total protein stains as loading controls: an alternative to high-abundance single-protein controls in semi-quantitative immunoblotting. J. Neurosci. Methods 172, 250-254, (2008) Abstract |
